ABSTRACT
Aim: This study was designed to evaluate the therapeutic potential of adipose tissue-derived mesenchymal stem cells (ATMSCs) compared to bone marrow-derived mesenchymal stem cells (BM-MSCs) on the regeneration of surgically createdcleft alveolus in dogs. Methods: Split mouth experimental study was performed on twelve healthy mongrel dogs. The dogswere divided into two groups (A and B): Ingroup A, the surgically created alveolus was transplanted with AT-MSCs,scaffold and growth factors at the experimental side (right side of the maxilla). Ingroup B, the surgically created alveoluswas transplanted with BM-MSCs, scaffold and growth factors at the experimental side (right side of the maxilla). In thecontrol side (left side of the maxilla), the surgically created alveolus was transplanted with scaffold and growth factors only.The flaps were replaced and sutured with resorbable sutures. Bone regeneration was evaluated clinically andradiographically after 1.5 and 3 months following dogs’ scarification. The data were evaluated with descriptive and t-testmethods (p=0.05). Results: Stem cells whether AT-MSCs or BM-MSCs accelerate the healing and regeneration of thedefected area by increasing the bone width and surface area; providing the bone quantity and quality as early as 1.5 and 3months. Conclusions: AT-MSCs and BM-MSCs are attractive tools in bone regeneration. AT-MSCs in experimental studiesshowed that their effectiveness is comparable to BM-MSCs, in addition to its low cost, ease of harvesting and saferprocedure to obtain stem cells as well as less risk of infection.
ABSTRACT
Aim: To evaluate the internal adaptation of some dental adhesive restorative material (Nano-composite resin /Biodentine,Nanocomposite resin / Nano- resin-modified glass ionomer and Nano-composite resin) to the primary dentinal surface usingmicro-computed tomography (Micro-CT) Materials and methods: Forty-five extracted primary molars, due to caries ororthodontic reasons, were collected provided that it has an intact buccal/ lingual surface(s) and one half to two-thirds ofroot length. The selected teeth were disinfected and stored in normal saline at room temperature. The teeth were randomlyassigned to one of the three experimental restorative groups according to the restoration type (15 per group): group A:Nanocomposite resin / Biodentine, group B: Nanocomposite resin / Nano-resin-modified glass ionomer and group C:restored totally with Nanocomposite resin. A high- resolution desktop micro-CT (Model 1172, Skyscan, Belgium) was usedto image the samples. Results: The mean rank of volumetric dimension values of the total gap at the restorative material –dentine interface demonstrated significant difference among the three groups (P= 0.003). Moreover, there was a significantdifference in the mean rank of the ratio of total gap volume/cavity volume among the three restorative groups (P=0.015).The data demonstrate that group A showed the lowest in total gap volume and mean ratio of total gap volume /cavity volumewhile group C recorded the highest value. Conclusion: Biodentine exhibited a higher internal adaptation to a dentinalsurface which is comparable to Nano resin-modified glass ionomer. The study results potentiate the importance of usingBiodentine liners under Nano-composite (sandwich technique) in terms of excellent internal adaptation, in addition to itshigh biocompatibility and easy handling as well.
ABSTRACT
To evaluate the effect of maternal xylitol consumption on children's salivary mutans streptococci [MS] level, caries activity, and plaque accumulation in contrast with maternal fluoride varnish in a group of mother-child pairs. In this randomized controlled trial, the study subjects were 60 mother-child pairs recruited from the pediatric dentistry clinic and the hospital well baby clinic at King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. The sample was recruited on the basis of maternal high salivary MS levels, and a child aged 10-36 months. The subjects were randomly assigned to experimental and control groups. Mothers in the experimental group chewed xylitol gum, 1.8 gram [66% xylitol by weight], 3 times/day for 3 months. Mothers in the control group received fluoride varnish. Both groups received oral hygiene instructions, dietary counseling, and restorative treatment. Children were examined after 6, 12, and 24 months from the initiation of the study to evaluate salivary MS levels, caries, and plaque accumulation. There was an increase in MS levels in the experimental and control children at 24 months, which was non-significant in the experimental group, and significantly higher in the control group when compared with the baseline [p=0.008]. The decayed, missing, filled scores of the children in the experimental group showed no change after 24 months, contrary to the controls that showed a significant increase [p=0.001]. Plaque scores revealed no differences over time or between the 2 groups. Compared with fluoride varnish, maternal xylitol consumption provided preventive outcomes on salivary MS and caries levels in children